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Brief introduction of rapid detecting method of glycated hemoglobin HbA1c

Glycated hemoglobin (HbA1c) is a gold standard of evaluation for long-term blood sugar control, but also an important basis for adjusting guidance of clinical treatment. Compared with the traditional diagnostic indicator(blood glucose) of diabetes, glycosylated hemoglobin (HbA1c) has small biological variability, it is less susceptible to fluctuation of blood glucose, it doesn't need to take blood in fasting or specific time, and it is more stable in advance of blood analysis.

In clinical laboratory, there are mainly two categories for rapid detecting methods of glycated hemoglobin at present: one category, based on charge difference of non-glycosylated hemoglobin & glycosylated hemoglobin, like ion chromatography, and electrophoresis, etc. and another category, based on structure and characteristic of glycated group in hemoglobin, such as affinity chromatography, ion capturing and immunization, etc. For our company's "QUO-LAB glycated hemoglobin a nalyzer", we will briefly introduce principle and technical characteristic of detecting glycated hemoglobin.

Affinity chromatography, is an affinity adsorption system which utilizes the principle of reversible binding of biological macromolecules with corresponding specific ligand molecule, and ligands are firmly bound to solid phase vehicle. In certain condition, target macromolecule (with impurities) isolates are capable of binding ligands with same phase by secondary bond, while the impurities are not absorbed. After removing impurities, we change the condition to dissociate the separated macromolecules, and obtain purified macromolecules. The vehicle of GHb affinity chromatography is an amino phenyl borate agarose gel; when the all GHb pass through the vehicle, cis-disaccharide alcohol portion containing glucose in the surface of stable GHb molecules, showed a specific covalent binding with phenyl borate ligand in stationary phase. Other non-glycated Hb, unstable GHb and HbF etc. will flow out with the mobile phase (asparagine buffer), and then we will use another mobile phase (sorbitol buffer) containing sugar or alkanes compounds to wash off GHb, and utilize the color value of two-part Hb to measure and calculate GHb which is detected by affinity chromatography in 415 nm.

The company's "QUO-LAB glycated hemoglobin analyzer" is a revolutionary rapid glycated hemoglobin analyzer. Through boric acid affinity chromatography, such analyzer just needs 4 ul whole blood to separate and detect glycated hemoglobin within 4 min, it can provide instant test result for clinic, so doctors can make a diagnosis in a short time, and make corresponding treatment plan for patients; such analyzer is particularly suitable for the use in clinical departments. The test result of the analyzer fully meet and exceed clinical requirements, CV value is less than 3%, which is leading level of market. The device is easy to operate, fast, low cost, specific, and difficult to be interfered by abnormal hemoglobin. It also has miniaturization characteristic. Experiments show that GHb value detected by the analyzer is highly correlated with HbA & HbAc &blood sugar detected by HPLC, while blood sugar should be detected in fasting and postprandial 2 h condition by HPLC. These results indicate that the method for diabetic patients is an ideal condition monitoring program.
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